Restoration of β-galactosidase to Escherichia coli M15. Complementation studies
نویسندگان
چکیده
منابع مشابه
Protein Interactions in Live Cells Monitored by β-Galactosidase Complementation
The characterization of protein interactions is important to the understanding of signal transduction pathways and cellular processes. Here we describe a method utilizing β-galactosidase (βgal) complementation that can monitor protein interactions in live mammalian cells (Rossi et al. 1997, 2000; Blakely et al. 2000). In brief, the method involves expressing chimeric proteins consisting of two ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1977
ISSN: 0264-6021
DOI: 10.1042/bj1650417c